Active GLP-1 (7-36) amide Sample Collection and Handling Guide

Glucagon-like peptide-1 (GLP-1) is a 30-amino acid incretin hormone. In response to nutrient intake, active GLP-1 (7-36) amide is secreted from intestinal L-cells and is rapidly degraded by dipeptidyl peptidase-4 (DPP-4). As a result, only 10-15% of the originally secreted active GLP-1 (7-36) amide reaches peripheral organs. Consequently, the active GLP-1 (7-36) amide is present at low physiological concentrations, making measurement extremely difficult, particularly in fasted subjects.

The proper collection and handling of GLP-1 samples is vital for the elimination of analytical variability and improved accuracy when measuring active GLP-1 (7-36) amide levels.

Download our guide to review sample collection, handling, and storage procedures that can help reduce technical errors and improve assay results when quantifying active GLP-1 (7-36) amide.