STELLUX® Solution

Platform Benefits

STELLUX® was introduced to the market in April 2013 as our chemiluminescent assay platform. The name is a term that merges the Latin words for “star” and “light” to describe the enzyme activated chemical reaction that occurs through chemiluminescence, literally bringing test results to light out of darkness. All of the STELLUX® chemiluminescent immunoassays were developed with life science researchers in mind. These ELISAs save laboratories time and money while achieving the highest level of performance.

All of our STELLUX® chemiluminescent immunoassays are highly sensitive, accurate broad range ELISAs that save laboratories time and money by significantly reducing sample preparation and taking the guesswork out of determining ideal dilution factors.

Key Features

  • Broad dynamic range
  • Superior sensitivity
  • Small sample volume
  • Performance data, including lot validation package

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Chemi Active GLP-1 ELISA

The STELLUX® Chemi Active GLP-1 ELISA (7-36 amide) is intended for the quantitative determination of active GLP- 1 (7-36 amide) in serum and plasma samples collected in the presence of DPP-IV inhibitor.

Benefits

Low sample volume

Requires only 10 µL of sample, 50% less than other commercial assays

Superior assay sensitivity

<1 pM, effective in quantifying fasted levels

No sample extraction

No matrix effects, no cross reactivity to pro-glucagon derived fragments

Product Specifications

Catalog #:80-GLP1A-CH01
Species:Human, Mouse, Rat
Sample Size:10 µL
Sample Type:EDTA Plasma, Serum
Range:2.25 - 3452 pg/mL*
Sensitivity:< 1 pM (3.3 pg/mL)
Incubation:2 hrs 22 min
*Standard is lyophilized and value assigned.

Fasted and Fed Active GLP-1 Levels in Mice

The assay detected differences between fasted and fed rodent sample groups showing changes in Active GLP-1 levels. Plasma was collected in the presence of DPP-4 inhibitor and/or Aprotinin.

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Fasted and Fed Active GLP-1 Levels in Rats

The assay detected differences between fasted and fed rodent sample groups showing changes in Active GLP-1 levels. Plasma was collected in the presence of DPP-4 inhibitor and/or Aprotinin.

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Dilutional Linearity

Sample types include: mouse serum, mouse EDTA plasma, rat serum, rat EDTA plasma and human EDTA plasma.

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Spike & Recovery

Sample types include: rat serum, rat EDTA plasma, mouse serum, mouse EDTA plasma and human EDTA plasma.

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Chemi Human C-peptide ELISA

The STELLUX® Human Chemi C-peptide ELISA measures human C-peptide in serum, plasma and cell culture samples. This highly characterized assay exhibits high sensitivity, broad dynamic range and is ideal for measuring human C-peptide in both Type 1 and Type 2 diabetes subjects.

Benefits

Broad dynamic range

Broad dynamic range to eliminate dilutions

No cross reactivity

No cross reactivity to human insulin or proinsulins or to rodent proinsulins

FDA Registered

FDA Registered for In Vitro Diagnostic Use

Product Specifications

Catalog #:80-CPTHU-CH01
Species:Human
Sample Size:50 µL
Sample Type:Plasma, Serum, Cell Culture
Range:4.32 – 12,960 pg/mL*
Sensitivity:≤4.32 pg/mL
Incubation:3 hrs
Controls:3 control levels included
*Lot dependent as standards are lyophilized and value assigned.

Performance Specifications

The assay uses 50 μL of sample and provides a broad detection range of 4.32 to 12,960 pg/mL* with an analytical sensitivity of ≤4.32 pg/mL.

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Inter-Laboratory Correlation

10 human samples (T1DM, T2DM and Normal serum) were evaluated at three independent laboratories. The results from each of the laboratories were compared to results generated at ALPCO, demonstrating a high degree of correlation.

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Comparison of Chemiluminescent and Colorimetric Assays with T1DM Samples

49 T1DM human serum samples were evaluated against a leading colorimetric assay. Of the 49 samples tested, 25 were detectable in the STELLUX® Assay and 15 were detectable in the colorimetric assay. This represents a >60% increase in sample detection when using the Human Chemi C-peptide ELISA.

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C-peptide Profiles in Serum Samples

The Human Chemi C-peptide ELISA was used to elucidate the different profiles of C-peptide levels in Normal (Fasted and Fed), T1DM, and T2DM (Fasted and Fed) human samples. The profiles clearly differentiate normal individuals and individuals with diabetes.

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Chemi Human HMW & Total Adiponectin ELISA

The highly specific STELLUX® Chemi Human HMW & Total Adiponectin ELISA provides researchers studying HMW/total ratios in type 2 diabetes, metabolic syndrome, obesity, coronary artery disease and non-alcoholic fatty liver disease with a tool to confidently measure adiponectin.

Benefits

High sensitivity

High sensitivity of ≤0.1 ng/mL

Specificity

Specificity of different adiponectin isoforms characterized using FPLC

No cross reactivity

No cross reactivity to human leptin, resistin, IL-6 or TNF-α

Product Specifications

Catalog #:80-ADPHU-CH01
Species:Human
Sample Size:5 µL
Sample Types:Plasma, Serum
Range:0.073 - 300 ng/mL
Sensitivity:≤0.1 ng/mL
Incubation:3 hrs 2 min
Controls:3 control levels included
Chemi Human Insulin ELISA

The STELLUX® Chemi Human Insulin ELISA measures the concentration of insulin in human serum, heparin plasma and tissue culture supernatants in the range of 5 – 30,000 pg/mL.

Benefits

Superior Sensitivity

Superior assay sensitivity ideal for measuring normal to pre-diabetic samples.

Shorter Incubation

20-40% shorter incubation than other assays on the market.

Dynamic Range

Broad dynamic range eliminates dilution steps.

No Cross-reactivity

No cross-reactivity to mouse and rat insulin.

Small Sample Size

Small sample size for preserving precious samples.

Product Specifications

Catalog #:80-INSHU-CH01
Species:Human
Sample Type:Plasma, Serum, Cell Culture
Sample Size:25 µL
Range:5 - 30,000 pg/mL
Sensitivity:2 pg/mL
Incubation:1 hr 35 min
Controls:3 control levels included

Performance Specifications

The STELLUX® Chemi Human Insulin ELISA provides a broad detection range of 5- 30,000 pg/mL (0.14 - 862 μIU/mL) with an analytical sensitivity of 2 pg/mL and functional sensitivity of 20 pg/mL using only a 25 μL sample.

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Precision & Correlation

Intra- and inter-assay precision values expressed less than 13.5% variation. Inter-laboratory testing demonstrated a high degree of correlation with an r2 value of 0.999.

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Additionally, correlation between the ALPCO human insulin colorimetric ELISA assays and the STELLUX® human insulin chemiluminescent platform was evaluated. Results showed a high degree of correlation with r2 values of 0.97 and 0.95.

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Fasted & Fed Samples

Chemi Human Intact Proinsulin ELISA

The STELLUX® Chemi Human Intact Proinsulin ELISA measures the concentration of intact proinsulin in human plasma, serum and tissue culture supernatants.

Benefits

Superior Sensitivity

Superior assay sensitivity is ideal for measuring normal to pre-diabetic proinsulin.

Highly Characterized

Highly characterized assay with <1% cross-reactivity to des (31,32) proinsulin.

Reduce Preparation Time

Reduce sample preparation time with broad dynamic range of 5 – 3,000 pg/mL.

Product Specifications

Catalog #:80-PINHUI-CH01
Species:Human and Non-Human Primate
Sample Type:Human Plasma, Serum
Sample Size:50 µL
Range:5 - 3,000 pg/mL
Sensitivity:<2.0 pg/mL
Incubation:2 hr 31 min
Controls:3 control levels included

Performance Specifications

The assay provides a broad detection range of 5-3,000 pg/mL with an analytical sensitivity of <2.0 pg/mL and functional sensitivity of 5 pg/mL using only a 50 μL sample.

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Specificity

The STELLUX® Human Intact Proinsulin ELISA demonstrates <1% cross reactivity with Des(31,32) proinsulin, ensuring only intact proinsulin is detected.

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Inter-Laboratory Correlation

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Internal Results

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Chemi Human Total Proinsulin ELISA

The STELLUX® Chemi Human Total Proinsulin ELISA offers superior sensitivity compared to other kits in the market. The assay measures intact as well as Des (31,32) and Des (64,65) proinsulin.

Benefits

Sensitivity

10x more sensitive than competitors, ideal for measuring normal to pre-diabetic samples.

Broad Dynamic Range

Broad dynamic range eliminates dilution steps

Highly Characterized

Highly characterized assay fully cross-reactive to des (31,32) & des (64,65) proinsulin.

Product Specifications

Catalog #:80-PINHUT-CH01
Species:Human
Sample Type:Plasma, Serum, Cell Culture
Sample Size:50 µL
Range:5 - 3,000 pg/mL
Sensitivity:0.455 pg/mL
Incubation:2 hr 31 min
Controls:3 control levels included

Performance Specifications

The assay provides a broad detection range of 5 - 3,000 pg/mL with an analytical sensivity of 0.455 pg/mL and functional sensitivity of 10 pg/mL using only a 50 μL sample.

Standard Curve

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Specificity

The STELLUX® Human Total Proinsulin ELISA fully detects des(31,32) and des(64,65) proinsulin, ensuring all circulating forms of proinsulin are measured in a single assay.

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Inter-Laboratory Correlation

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Unparallelled Sensitivity

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Chemi Rodent Insulin ELISA

The STELLUX® Chemi Rodent Insulin ELISA measures the concentration of the insulin protein products of the mouse I, mouse II, rat I and rat II proinsulin genes. The kit has the performance characteristics and flexibility necessary to confidently measure up to 36 samples in duplicate.

Benefits

Dynamic Range

Broad dynamic range eliminates dilution steps.

Small Sample Size

Small sample size is ideal for rodent models.

Short Incubation

Short incubation, saves time.

Product Specifications

Catalog #:80-INSMR-CH01
Species:Mouse, Rat
Sample Type:Plasma, Serum, Cell Culture
Sample Size:5 µL
Range:0.1-150 ng/mL
Sensitivity:0.096 ng/ml
Incubation:2hr
Controls:3 control levels included

Performance Specifications

The assay provides a broad detection range of 0.1 – 150 ng/mL with an analytical sensitivity of 0.096 ng/mL and functional sensitivity of 0.15 ng/mL using only a 5 µL sample.

Standard Curve

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Precision & Correlation

Intra- and inter-assay precision values expressed less than 13.5% variation. Inter-laboratory testing demonstrated a high degree of correlation with an r2 value of 0.999.

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Fasted & Fed Samples

Chemi Ultrasensitive Active GLP-1 ELISA

The Chemi Ultrasensitive Active GLP-1 ELISA is the most sensitive assay on the market which only requires 25 μL of sample. The assay consistently displays precision and accuracy through the elimination of matrix interference, illustrated by extensive dilutional linearity and spike and recovery studies. The enhanced sensitivity is ideal for measuring fasted levels of GLP-1 in rats and mice.

Benefits

2x greater sensitivity

Ideal for quantifying fasted levels.

50% less sample volume

Ideal for measuring rodent samples.

No sample extraction, matrix effects, or cross-reactivity

No cross-reactivity to pro-glucagon derived fragments.

Product Specifications

Catalog #:80-GLP1AU-CH01
Species:Human, Mouse, Rat
Sample Type:EDTA Plasma (Human, Rodent), Serum (Rodent)
Sample Size:25 µL
Range:0.76 - 1,036 pg/mL
Sensitivity:<0.33 pg/mL
Incubation:2 hr 22 min

Dilutional Linearity

Sample types include: mouse serum, mouse EDTA plasma, rat serum, rat EDTA plasma and human EDTA plasma.

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Specificity

In order to demonstrate that our assay is specific for the (7-36) amide form of GLP-1, we tested crossreactivity against the following GLP-1 species.

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Spike & Recovery

Sample types include: mouse serum, mouse EDTA plasma, rat serum, rat EDTA plasma and human EDTA plasma.

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Competitive Comparison

The Chemi Ultrasensitive Active GLP-1 ELISA is the most sensitive assay on the market requiring only 25 μL of sample.

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Chemiluminescent Plate Reader

The STELLUX® Chemiluminescent Plate Reader was designed and developed to provide researchers with an affordable, versatile, and user-friendly system for running and analyzing chemiluminescent ELISAs.

Benefits

User Friendly

Simple "plug-and-play" set up.

Ideal Size

For use in any laboratory.

Buit-in Software

Capable of performing both 4 and 5 parameter curve fits.

Product Specifications

Catalog #:85-STX4400
Detection Mode:Glow luminescence
Sensitivity/Detection Limit:HRP 1 x 10-18 moles, ALP 1 x 10-21 moles
Linear Dynamic Range:106 RLU
Cross-talk:Less than 2.5 x 10-4
Detector:Photomultiplier (PMT)
Spectral Response Range:300-650 nm
Peak Wavelength:400 nm
Vessel:96 wells in opaque white, flat bottom wells in a strip tray or plate

Data Analysis

The STELLUX® Plate Reader system is a compact, PC-controlled open chemiluminescent system designed to read and calculate the results of assays read in 96-well microtiter plates or strip trays. The reader’s built-in data analysis software allows users to create a menu of assay templates for instant recall with these features:
- Selectable plate formatting
- Alphanumeric test naming
- Automatic interpretation options
- Duplicate well options
- Curve plotting and editing
- Flags and error messages

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The Ultimate Stellar Solution

Unlike other test platforms, ALPCO’s line of chemiluminescent assays does not require expensive high-end instrumentation. The STELLUX® ELISAs are 96-well black plate assays requiring a standard chemiluminescent (glow) reader.

The STELLUX® Chemiluminescent Plate Reader was developed as an affordable solution that only occupies a small amount of valuable laboratory bench space. The STELLUX® Chemiluminescent ELISAs and Plate Reader are ideal for use in academic institutions, contract research organizations, core reference laboratories, and pharmaceutical companies.

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Related Resources

Brands

Our commitment to support the advancement of research & clinical outcomes led us to develop our STELLUX® Chemiluminescence and Spectru™ Flow Cytometry brands.

Stellux Logo

STELLUX® was introduced to the market in April 2013 as our chemiluminescent assay platform. The name is a term that merges the Latin words for “star” and “light” to describe the enzyme activated chemical reaction that occurs through chemiluminescence, literally bringing test results to light out of darkness.

Learn More
Spectru™ Logo

Spectru™ was established in 2013 as our signature brand of flow cytometry products. The name is a coined term that abbreviates the word “spectrum”, referring to the visual properties of flow cytometry testing, in a way that reveals the word “true” which comments on the clarity of finding results. Also sounding like the word “specter,” Spectru™ embodies the idea of revealing information that was initially invisible.

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