STELLUX® Solution
Platform Benefits
STELLUX® was introduced to the market in April 2013 as our chemiluminescent assay platform. The name is a term that merges the Latin words for “star” and “light” to describe the enzyme activated chemical reaction that occurs through chemiluminescence, literally bringing test results to light out of darkness. All of the STELLUX® chemiluminescent immunoassays were developed with life science researchers in mind. These ELISAs save laboratories time and money while achieving the highest level of performance.
All of our STELLUX® chemiluminescent immunoassays are highly sensitive, accurate broad range ELISAs that save laboratories time and money by significantly reducing sample preparation and taking the guesswork out of determining ideal dilution factors.
Key Features
- Broad dynamic range
- Superior sensitivity
- Small sample volume
- Performance data, including lot validation package
Shop STELLUX®
The STELLUX® Chemi Active GLP-1 (7-36) amide ELISA is intended for the quantitative determination of active GLP- 1 (7-36) amide in human, mouse, and rat EDTA plasma samples collected in the presence of DPP-4 inhibitor.
Benefits
Small Sample Volume
Requires only 25 µL
Highly Specific
No cross-reactivity to other forms of GLP-1
Reliably Detect Low Fasted Levels
Functional sensitivity: 0.45 pM Analytical sensitivity: 0.15 pM
Product Specifications
Catalog #: | 80-GLP1A-CH01 |
Species: | Human, Mouse, Rat |
Sample Type: | EDTA Plasma |
Sample Size: | 25 µL |
Range: | 0.45-151.6 pM |
Sensitivity (LOD): | 0.15 pM |
Incubation: | 2 hr 52 min |
Controls: | 3 |
Analytical Specificity
Unlike many commercially available active GLP-1 ELISAs, the STELLUX® Chemi Active GLP-1 (7-36) amide ELISA is 100% specific to the bioactive form of GLP-1 with no cross-reactivity to other forms or proglucagon peptides.

Spike and Recovery
The STELLUX® Chemi Active GLP-1 (7-36) amide ELISA offers the versatility to be used in rodent and human models. Mouse, rat, and human samples were spiked with GLP-1 and measured to demonstrate recovery in each matrix.

Cross-reactivity by Analyte
The table below indicates the analyte and cross-reactivity percentage to other forms of GLP-1 and proglucagon peptides observed in the assay.

The STELLUX® Human Chemi C-peptide ELISA measures human C-peptide in serum, plasma and cell culture samples. This highly characterized assay exhibits high sensitivity, broad dynamic range and is ideal for measuring human C-peptide in both Type 1 and Type 2 diabetes subjects.
Benefits
Broad dynamic range
Broad dynamic range to eliminate dilutions
No cross reactivity
No cross reactivity to human insulin or proinsulins or to rodent proinsulins
FDA Registered
FDA Registered for In Vitro Diagnostic Use
Product Specifications
Catalog #: | 80-CPTHU-CH01 |
Species: | Human |
Sample Size: | 50 µL |
Sample Type: | Plasma, Serum, Cell Culture |
Range: | 4.32 – 12,960 pg/mL* |
Sensitivity: | ≤4.32 pg/mL |
Incubation: | 3 hrs |
Controls: | 3 control levels included |
*Lot dependent as standards are lyophilized and value assigned. |
Performance Specifications
The assay uses 50 μL of sample and provides a broad detection range of 4.32 to 12,960 pg/mL* with an analytical sensitivity of ≤4.32 pg/mL.

Inter-Laboratory Correlation
10 human samples (T1DM, T2DM and Normal serum) were evaluated at three independent laboratories. The results from each of the laboratories were compared to results generated at ALPCO, demonstrating a high degree of correlation.

Comparison of Chemiluminescent and Colorimetric Assays with T1DM Samples
49 T1DM human serum samples were evaluated against a leading colorimetric assay. Of the 49 samples tested, 25 were detectable in the STELLUX® Assay and 15 were detectable in the colorimetric assay. This represents a >60% increase in sample detection when using the Human Chemi C-peptide ELISA.

C-peptide Profiles in Serum Samples
The Human Chemi C-peptide ELISA was used to elucidate the different profiles of C-peptide levels in Normal (Fasted and Fed), T1DM, and T2DM (Fasted and Fed) human samples. The profiles clearly differentiate normal individuals and individuals with diabetes.

The STELLUX® Chemi Human Insulin ELISA measures the concentration of insulin in human serum, heparin plasma and tissue culture supernatants in the range of 5 – 30,000 pg/mL.
Benefits
Superior Sensitivity
Superior assay sensitivity ideal for measuring normal to pre-diabetic samples.
Shorter Incubation
20-40% shorter incubation than other assays on the market.
Dynamic Range
Broad dynamic range eliminates dilution steps.
No Cross-reactivity
No cross-reactivity to mouse and rat insulin.
Small Sample Size
Small sample size for preserving precious samples.
Product Specifications
Catalog #: | 80-INSHU-CH01 |
Species: | Human |
Sample Type: | Plasma, Serum, Cell Culture |
Sample Size: | 25 µL |
Range: | 5 - 30,000 pg/mL |
Sensitivity: | 2 pg/mL |
Incubation: | 1 hr 35 min |
Controls: | 3 control levels included |
Performance Specifications
The STELLUX® Chemi Human Insulin ELISA provides a broad detection range of 5- 30,000 pg/mL (0.14 - 862 μIU/mL) with an analytical sensitivity of 2 pg/mL and functional sensitivity of 20 pg/mL using only a 25 μL sample.

Precision & Correlation
Intra- and inter-assay precision values expressed less than 13.5% variation. Inter-laboratory testing demonstrated a high degree of correlation with an r2 value of 0.999.

Additionally, correlation between the ALPCO human insulin colorimetric ELISA assays and the STELLUX® human insulin chemiluminescent platform was evaluated. Results showed a high degree of correlation with r2 values of 0.97 and 0.95.

Fasted & Fed Samples

The STELLUX® Chemi Human Intact Proinsulin ELISA measures the concentration of intact proinsulin in human plasma, serum and tissue culture supernatants.
Benefits
Superior Sensitivity
Superior assay sensitivity is ideal for measuring normal to pre-diabetic proinsulin.
Highly Characterized
Highly characterized assay with <1% cross-reactivity to des (31,32) proinsulin.
Reduce Preparation Time
Reduce sample preparation time with broad dynamic range of 5 – 3,000 pg/mL.
Product Specifications
Catalog #: | 80-PINHUI-CH01 |
Species: | Human and Non-Human Primate |
Sample Type: | Human Plasma, Serum |
Sample Size: | 50 µL |
Range: | 5 - 3,000 pg/mL |
Sensitivity: | <2.0 pg/mL |
Incubation: | 2 hr 31 min |
Controls: | 3 control levels included |
Performance Specifications
The assay provides a broad detection range of 5-3,000 pg/mL with an analytical sensitivity of <2.0 pg/mL and functional sensitivity of 5 pg/mL using only a 50 μL sample.

Specificity
The STELLUX® Human Intact Proinsulin ELISA demonstrates <1% cross reactivity with Des(31,32) proinsulin, ensuring only intact proinsulin is detected.

Inter-Laboratory Correlation

Internal Results

The STELLUX® Chemi Human Total Proinsulin ELISA offers superior sensitivity compared to other kits in the market. The assay measures intact as well as Des (31,32) and Des (64,65) proinsulin.
Benefits
Sensitivity
10x more sensitive than competitors, ideal for measuring normal to pre-diabetic samples.
Broad Dynamic Range
Broad dynamic range eliminates dilution steps
Highly Characterized
Highly characterized assay fully cross-reactive to des (31,32) & des (64,65) proinsulin.
Product Specifications
Catalog #: | 80-PINHUT-CH01 |
Species: | Human |
Sample Type: | Plasma, Serum, Cell Culture |
Sample Size: | 50 µL |
Range: | 5 - 3,000 pg/mL |
Sensitivity: | 0.455 pg/mL |
Incubation: | 2 hr 31 min |
Controls: | 3 control levels included |
Performance Specifications
The assay provides a broad detection range of 5 - 3,000 pg/mL with an analytical sensivity of 0.455 pg/mL and functional sensitivity of 10 pg/mL using only a 50 μL sample.
Standard Curve

Specificity
The STELLUX® Human Total Proinsulin ELISA fully detects des(31,32) and des(64,65) proinsulin, ensuring all circulating forms of proinsulin are measured in a single assay.

Inter-Laboratory Correlation

Unparallelled Sensitivity

The STELLUX® Chemi Rodent Insulin ELISA measures the concentration of the insulin protein products of the mouse I, mouse II, rat I and rat II proinsulin genes. The kit has the performance characteristics and flexibility necessary to confidently measure up to 36 samples in duplicate.
Benefits
Dynamic Range
Broad dynamic range eliminates dilution steps.
Small Sample Size
Small sample size is ideal for rodent models.
Short Incubation
Short incubation, saves time.
Product Specifications
Catalog #: | 80-INSMR-CH01 |
Species: | Mouse, Rat |
Sample Type: | Plasma, Serum, Cell Culture |
Sample Size: | 5 µL |
Range: | 0.1-150 ng/mL |
Sensitivity: | 0.096 ng/ml |
Incubation: | 2hr |
Controls: | 3 control levels included |
Performance Specifications
The assay provides a broad detection range of 0.1 – 150 ng/mL with an analytical sensitivity of 0.096 ng/mL and functional sensitivity of 0.15 ng/mL using only a 5 µL sample.
Standard Curve

Precision & Correlation
Intra- and inter-assay precision values expressed less than 13.5% variation. Inter-laboratory testing demonstrated a high degree of correlation with an r2 value of 0.999.

Fasted & Fed Samples



The STELLUX® Chemiluminescent Plate Reader was designed and developed to provide researchers with an affordable, versatile, and user-friendly system for running and analyzing chemiluminescent ELISAs.
Benefits
User Friendly
Simple "plug-and-play" set up.
Ideal Size
For use in any laboratory.
Product Specifications
Catalog #: | 85-STX4400 |
Detection Mode: | Glow luminescence |
Sensitivity/Detection Limit: | HRP 1 x 10-18 moles, ALP 1 x 10-21 moles |
Linear Dynamic Range: | 106 RLU |
Cross-talk: | Less than 2.5 x 10-4 |
Detector: | Photomultiplier (PMT) |
Spectral Response Range: | 300-650 nm |
Peak Wavelength: | 400 nm |
Vessel: | 96 wells in opaque white, flat bottom wells in a strip tray or plate |
The Ultimate Stellar Solution
Unlike other test platforms, ALPCO’s line of chemiluminescent assays does not require expensive high-end instrumentation. The STELLUX® ELISAs are 96-well black plate assays requiring a standard chemiluminescent (glow) reader.
The STELLUX® Chemiluminescent Plate Reader was developed as an affordable solution that only occupies a small amount of valuable laboratory bench space. The STELLUX® Chemiluminescent ELISAs and Plate Reader are ideal for use in academic institutions, contract research organizations, core reference laboratories, and pharmaceutical companies.

Related Resources
Brands
Our commitment to support the advancement of research & clinical outcomes led us to develop our STELLUX® Chemiluminescence and Spectru™ Flow Cytometry brands.
STELLUX® was introduced to the market in April 2013 as our chemiluminescent assay platform. The name is a term that merges the Latin words for “star” and “light” to describe the enzyme activated chemical reaction that occurs through chemiluminescence, literally bringing test results to light out of darkness.
Learn MoreSpectru™ was established in 2013 as our signature brand of flow cytometry products. The name is a coined term that abbreviates the word “spectrum”, referring to the visual properties of flow cytometry testing, in a way that reveals the word “true” which comments on the clarity of finding results. Also sounding like the word “specter,” Spectru™ embodies the idea of revealing information that was initially invisible.
Learn More