Direct Saliva Melatonin ELISA
The Direct Saliva Melatonin ELISA kit is intended for the quantitative determination of melatonin in human saliva samples. Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Available in North America Only
The Direct Saliva Melatonin ELISA is a competitive immunoassay using a capture antibody (Ab) technique. The polyclonal Kennaway G280 anti-melatonin antibody (5, 6) has been coated onto the microtiter plate, provided in the kit. After the first 16-20-hours overnight incubation, melatonin present in the pre-treated saliva and controls as well as in the calibrators, compete with biotinylated melatonin during a 3 hour incubation for the binding sites of this highly specific antibody. After washing, the enzyme label streptavidin conjugated to horseradish peroxidase (HRP) is added, which binds to the melatonin-biotinantibody complexes captured on the coated wells during a 60-minute incubation step. Unbound enzyme label is then removed by a washing step and TMB substrate (tetramethylbenzidine) is added to the wells. In a further 30- minute incubation step, a chromophore is formed in inverse proportion to the amount of melatonin present in the sample. The color turns from blue to yellow after the addition of an acidic stop solution and can be measured at 450 nm. See instructions for use for references.