The STELLUX® Chemi Human Total Proinsulin ELISA offers superior sensitivity compared to other kits in the market. The assay measures intact as well as Des (31,32) and Des (64,65) proinsulin.
The Proinsulin (Total) Chemiluminescence ELISA is a sequential sandwich assay, which uses two mouse monoclonal antibodies: one as capture that is adsorbed to a 96-well black microplate and the other as a primary detector conjugated to biotin. Standards, sample, and controls are added to the plate and incubated at room temperature on a plate shaker set between 700-900 rpm. Post standard/sample incubation, the plate is washed six times and the biotinylated detection antibody is added to each well. This capture-antigen-detector sandwich is incubated at room temperature on a plate shaker set between 700-900 rpm. Post detection antibody incubation, the plate is washed six times, HRP (horseradish peroxidase)-conjugated streptavidin (SA) is added to each well and incubated at room temperature on a plate shaker set between 700-900 rpm. Post SA-HRP incubation, the plate is washed 6 times and the chemiluminescent substrate is added to each well. The microplate is analyzed on an appropriate plate reader 1 minute post substrate. The resulting relative light units (RLUs) are directly proportional to the amount of proinsulin present in the sample.
1. What percentage of your IgE allergy testing panel consists of molecular allergens rather than allergen extracts? The ALEX2 IgE allergen panel consists of 117 extracts and 183 molecular allergens. This represents the largest panel of molecular allergens on the market, including 50 unique...
Cases of food and environmental allergies have been increasing over the last decade. While conservative estimates suggest IgE-mediated allergy affects 6-8% of children and 2-3% of adults, some estimates are as high as 30%.3, 2 Symptoms can range from mild to severe and include itching, hives,...
Food sensitivity or food intolerance involves a non-allergic response to a food or food additive. In a recent on-line survey conducted in the U.S., 24% of adults aged 18 to 79 reported a food intolerance. While a food allergy is typically mediated by IgE antibodies, adverse reactions to food...
Salem, NH, December 19, 2023 – Revvity’s EUROIMMUN business, a leading provider of high-quality in-vitro diagnostic products, and ALPCO-GeneProof, a global leader in molecular diagnostics, jointly announced a strategic partnership to enhance the availability of GeneProof PCR kits throughout the...
ALPCO offers targeted GI solutions suitable for all types of labs. Our offerings range from plate-based to clinical chemistry analyzer-based and automated flash chemiluminescence solutions, all designed to accommodate your lab’s expanding testing needs and capabilities.
In the webinar, Dr. Benjamin Click from the University of Colorado, Department of Gastroenterology, underscores the critical role of non-invasive monitoring methods, particularly fecal calprotectin monitoring, in guiding treatment adjustments and achieving therapeutic goals..
Energy homeostasis is a focus of research into diabetes and obesity. Read about how our assays in this area can assist both academic and industry researchers.
Complement System Solutions for Drug Development and Clinical Research Datasheet
The complement system is essential to innate immunity from a variety of diseases. ALPCO’s full range of complement system immunoassays can drive discovery.
Melatonin Products for Sleep Disorder-Related Research Datasheet
The melatonin hormone affects the circadian rhythm and sleep-wake cycle. Find out about our immunoassays and products that explore melatonin and sleep disorders.