The Plasma/Serum Melatonin ELISA (12-MLTN-PL-U) is intended for the highly sensitive, quantitative determination of melatonin in plasma, serum, urine, and other biological fluids. The kit includes 20 extraction columns. Each extraction column provided with this kit can be used up to five times according to the extraction procedures in the product IFU. Additional extraction columns are available separately (catalog# 12-B-MECX).
For research use only. Not intended for use in diagnostic procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
0.70 - 50 pg/mL
Sensitivity
0.70 pg/mL
Sizes
96 Wells
Sample Types
Plasma, See Package Insert, Serum, Urine
Inc Time Overnight
Yes
Inc Time See Protocol
No
Sample Size
0.25
Detection
Colorimetric
The Plasma/Serum Melatonin ELISA is a competitive immunoassay using a capture antibody technique. Melatonin present in extracted serum, plasma or any other biological fluid samples and controls compete with biotinylated melatonin for the binding sites of the highly specific polyclonal Kennaway G280 anti-melatonin antibody during an overnight incubation. During this incubation the formed antibody-melatonin-biotin complexes are captured onto the pre-coated wells of the microplate. After washing away unbound melatonin-biotin conjugate the enzyme label (streptavidin conjugated to horseradish peroxidase) is added, which binds to the free biotin sites captured on the coated wells during a 60-minute incubation step. Unbound enzyme label is then removed by a second washing step and TMB (tetramethylbenzidine) substrate is added to the wells. In a further 30-minute incubation step, a chromophore is formed (turns from colorless to blue) in inverse proportion to the amount of melatonin originally present in the samples, controls and calibrators. The color turns from blue to yellow after the addition of an acidic stop solution and can be measured at 450 nm.
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