Rat Beta-2 Microglobulin ELISA
$902.00
Catalog
41-MICRT-E01
The Rat Beta-2 Microglobulin ELISA is for the quantitative determination of Microglobulin Beta-2 in rat Plasma or Serum.
The Rat B-2-Microglobulin ELISA can be used with plasma, serum and urine samples.
Recommended starting dilutions are as follows:
plasma, serum: 1/100
urine: 1/40
Research Use Only. Not for Use in Diagnostic Procedures.
The Rat B-2-Microglobulin ELISA can be used with plasma, serum and urine samples.
Recommended starting dilutions are as follows:
plasma, serum: 1/100
urine: 1/40
Research Use Only. Not for Use in Diagnostic Procedures.
Species
Rat
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
0.156 - 5 ng/ mL
Sensitivity
0.156 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum, Urine
Inc Time Hour
1
Inc Time Minute
30
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
In this assay the Beta 2-Microglobulin present in samples reacts with the anti-Beta 2-Microglobulin antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-B2M antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound B2M. Following another washing step, bound enzyme is assayed by the addition of a chromogenic substrate, 3,3’,5,5’- tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of B2M in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of B2M in the test sample. The quantity of B2M in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.