The Mouse/Rat Urocortin 3 ELISA is for the quantitative determination of urocortin in mouse or rat Plasma, Serum, or brain Tissue extract. Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
0.41 - 100 ng/mL
Sensitivity
0.137 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
25
Detection
Colorimetric
This EIA kit is used for quantitative determination of urocortin 3 in mouse/rat plasma, serum and their brain tissue extracts. The kit is characterized for sensitive quantification, high specificity and no influence with other components in samples. Mouse/rat urocortin 3 standard is highly purified synthetic product. < Specificity > This EIA kit has high specificity to mouse/rat urocortin 3 and shows no crossreactivity to urocortin 1 (mouse, rat), urocortin 1 (human), urocortin 2 (mouse), urocortin 2 (rat), ACTH (mouse, rat), ACTH (human) and CRF (mouse, rat, human). < Assay principle > This EIA kit for determination of mouse/rat urocortin 3 in samples is based on a competitive enzyme immunoassay using combination of highly specific antibody to mouse/rat urocortin 3 with biotin-avidin affinity system. To the wells of plate coated with rabbit anti mouse/rat urocortin 3 antibody, standards or samples and labeled antigen (biotinylated antigen) are added for competitive immunoreaction. After incubation and plate washing, horseradish peroxidase (HRP) labeled streptoavidin (SA) is added to form HRP labeled SA-labeled antigen-antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by o-Phenylenediamine dihydrochloride (OPD) and the concentration of mouse/rat urocortin 3 is calculated.
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