Glutathione Peroxidase Activity Assay (GPx Activity Assay)
$971.00
Catalog
30-7031
Glutathione Peroxidase Activity Assay (GPx Activity Assay)
Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
70 mg/mL
Sensitivity
see package insert
Sizes
96 Wells
Sample Types
Biological Fluids
Inc Time Hour
1
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
20
Glutathione peroxidase (GPx) is one of the most important enzymes for detoxification of peroxides in living cells. GPx plays a crucial role in protecting cells from damage by free radicals which are formed by peroxide decomposition. Lipid components of the cell are especially susceptible to reactions with free radicals resulting in lipid peroxidation. GPx reduces peroxides to alcohols using glutathione thus preventing the formation of free radicals. GPx enzymes catalyze the reduction of hydrogen peroxide and a wide variety of organic peroxides to water and the corresponding stable alcohols using cellular glutathione as the reducing reagent. Most cellular glutathione peroxidases are tetrameric enzymes consisting of four identical 22 kDa monomers, each of which contains a selenocysteine in the active site. The selenocysteine participates directly in electron donation to the peroxide substrate and becomes oxidized in the process. The enzyme then uses reduced glutathione as a hydrogen donor to regenerate the selenocysteine. GPx enzymes also exist as non-selenium (non-Se) containing enzymes. Cellular GPx is present in all tissues; however, various diseases may influence its level. An increase in the level of glutathione peroxidase has been observed in reticulocytes of diabetic rats. The level returned to normal after administration of insulin. A decrease in the level of the enzyme has been observed in patients suffering from diseases such as hairy cell leukemia or Favism (a disease associated with extreme hemolytic crisis).
The Glutathione Peroxidase Activity Assay (GPx Activity Assay) is for Research Use Only. The Glutathione Peroxidase Activity Assay (GPx Activity Assay) is not for use in diagnostic procedures.
For more information on the Glutathione Peroxidase Activity Assay (GPx Activity Assay), call 800-592-5726.
The Glutathione Peroxidase Activity Assay (GPx Activity Assay) is for Research Use Only. The Glutathione Peroxidase Activity Assay (GPx Activity Assay) is not for use in diagnostic procedures.
For more information on the Glutathione Peroxidase Activity Assay (GPx Activity Assay), call 800-592-5726.