The Alpha 1-Acid Glycoprotein test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Alpha 1-Acid Glycoprotein in biological samples of rats. Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
12.5 - 400 ng/mL
Sensitivity
12.5 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Hour
1
Inc Time Minute
20
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
In this assay the Alpha 1-Acid
Glycoprotein present in samples reacts with the anti-Alpha 1-Acid Glycoprotein antibodies which have been adsorbed
to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-AGP antibodies
conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with
the previously bound AGP. Following another washing step, the enzyme bound to the immunosorbent is assayed by
the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies
directly with the concentration of AGP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the
concentration of AGP in the test sample. The quantity of AGP in the test sample can be interpolated from the standard
curve constructed from the standards, and corrected for sample dilution.
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