Alpha-1 Antitrypsin ELISA
$524.00
Catalog
30-A1AHU-E01
The Alpha-1 Antitrypsin ELISA is intended for the quantitative determination of A1A in human stool samples. For research use only. Not for use in diagnostic procedures.
- Compatible with Easy Stool Extraction Device
- Highly specific polyclonal antibodies eliminate false negative readings
Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
3.3 - 90 ug/L
Sensitivity
3.3 ug/L
Sizes
96 Wells
Sample Types
Stool
Inc Time Hour
2
Inc Time Minute
20
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
15
Sample Size 2
100
Detection
Colorimetric
The assay utilizes the “sandwich” technique with two selected antibodies that bind to human α1- antitrypsin. Standards, controls, and prediluted samples, which are assayed for human α1-antitrypsin, are added into the wells of a microplate coated with a high affinity anti-human α1-antitrypsin antibody. During the first incubation step, α1-antitrypsin is bound by the immobilized antibody. Then a peroxidase-conjugated polyclonal anti-human α1-antitrypsin antibody is added into each microtiter well and a “sandwich” of capture antibody–human α1-antitrypsin–peroxidase-conjugate is formed. Tetramethylbenzidine is used as peroxidase substrate. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of α1-antitrypsin. A dose response curve of the absorbance (optical density, OD at 450nm) versus concentration is generated, using the values obtained from the standards. The α1-Antitrypsin present in the samples is determined directly from this curve.
