The Alpha 1-Antichymotrypsin ELISA kit is a highly sensitive two-site enzyme linked immunoassay for measuring alpha 1-antichymotrypsin in biological fluids of humans.
Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
6.25-400 ng/mL
Sensitivity
6.25 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Hour
1
Inc Time Minute
30
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
In this assay the
Alpha 1-Antichymotrypsin present in samples reacts with the anti-Alpha 1-Antichymotrypsin
antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-CYT antibodies conjugated with horseradish
peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the
previously bound CYT. Following another washing step, the enzyme bound to the immunosorbent
is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The
quantity of bound enzyme varies directly with the concentration of CYT in the sample tested; thus,
the absorbance, at 450 nm, is a measure of the concentration of CYT in the test sample. The
quantity of CYT in the test sample can be interpolated from the standard curve constructed from the
standards and corrected for sample dilution.
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