Free Testosterone ELISA

$239.00
Catalog
11-FTEHU-E01

The Free Testosterone ELISA is for the direct quantitative determination of Free Testosterone by enzyme immunoassay in human serum.

FDA Registered. For In Vitro Diagnostic Use.

Species
Human
Regulatory Status
FDA Registered. For In Vitro Diagnostic Use.
Product Distribution
Available Worldwide
Range
0.1 - 60 pg/mL
Sensitivity
0.1 pg/mL
Sizes
96 Wells
Sample Types
Serum
Inc Time Hour
1
Inc Time Minute
15
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
25
Detection
Colorimetric

The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of free testosterone in the sample. A set of standards is used to plot a standard curve from which the amount of free testosterone in patient samples and controls can be directly read. The ALPCO free testosterone kit utilizes a highly specifi c rabbit anti-testosterone polyclonal antibody at a low binding capacity (Keq x concentration) to keep minimum disturbances of the testosterone-protein equilibrium. The other components in the test system are also optimized in order to not alter the original free testosterone concentration.

Free Testosterone ELISA

11-FTEHU-E01

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