The Urinary Prostacyclin ELISA kit is a colorimetric competitive enzyme immunoassay kit for the quantitative determination of urinary prostacyclin metabolites in culture supernatants and urine from any species with results in < 3 hours. Absorbance is read at 405 nm. Quantitate PGI2 by measuring the stable metabolites in one simple assay.
Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
7.81 - 2,000 pg/mL
Sensitivity
6.58 pg/mL
Sizes
96 Wells
Sample Types
Cell Culture, Urine
Inc Time Hour
3
Inc Time Overnight
No
Inc Time See Protocol
No
Detection
Colorimetric
Prostacyclin (PGI2) is involved in platelet aggregation, vasoconstriction, and reproductive functions (1-3). PGI2 has a half life of 60 minutes in plasma but only 2 to 3 minutes in buffer (3). The production of PGI2 is typically monitored by measurement of 6k-PGF1α and 2,3d-6k-PGF1α. 6-keto-PGF1α is produced by the non-enzymatic hydration of PGI2, and is further metabolized in urine to the 2,3-dinor derivative. A number of pharmaceuticals alter and/or inhibit the synthesis of PGI2
(4). Methods to measure PGI2
in urine typically involve HPLC5
, gas chromatography/mass spectrometry (6), or radioimmunoassay (7,8)
and enzyme immunoassay (9) for 6k-PGF1α. Please see instructions for use for references.
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