This ELISA kit is intended for the quantitative detection of anti-Giardia lamblia IgG antibody in serum or plasma. This assay is a useful tool for detecting Giardia lamblia infections in acute or chronic gastroenteritis.
For research use only. Not for use in diagnostics procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Range
See insert
Sensitivity
see insert
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Hour
1
Inc Time Minute
30
Inc Time Overnight
No
Inc Time See Protocol
No
Detection
Colorimetric
The assay utilizes the microplate-based enzyme immunoassay
technique by coating highly purified and inactive Giardia lamblia
antigen onto the wall of microtiter well. Assay standards, controls and sample are added to
wells of a microplate coated with a highly purified Giardia lamblia antigen. The Giardia lamblia antigen will bind to the antibody in the liquid standards, controls, and samples. The unbound matrices are washed away and a HRP-conjugated antibody which specifically recognizes the specific
subtype of human antibody (IgG) is added for further
immunoreactions. After an incubation period, an immunocomplex of
“Giardia lamblia Antigen – human Anti-Giardia IgG- HRP-conjugated
Anti-hIgG Antibody” is formed if the human anti-Giardia IgG is
present. The unbound tracer antibody and other
protein or buffer matrix are removed in the subsequent washing step.
HRP-conjugated tracer antibody bound to the well is then incubated
with a substrate solution in a timed reaction and measured in a
spectrophotometric microplate reader. The enzymatic activity of the
tracer antibody bound to the wall of each microtiter well is directly
proportional to the amount of human Anti-Giardia lamblia IgG level in each sample.
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