The Direct Saliva Melatonin RIA kit is intended for the direct quantitative determination of melatonin in human saliva samples. Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
0.5 - 50 pg/mL
Sensitivity
0.3 pg/mL
Sizes
200 Tubes
Sample Types
Saliva
Inc Time Hour
20
Inc Time Minute
15
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
400
Detection
I-125
The Direct Saliva Melatonin RIA Kit measures melatonin by a double-antibody radio immunoassay based on the Kennaway G280 anti-melatonin antibody (5,6). Human saliva samples and reconstituted standards and controls are incubated with the anti-melatonin antibody and 125I-melatonin. 125I-melatonin competes with melatonin present in samples, standards and controls. After 20 hours of incubation, solid-phase second antibody is added to the mixture in order to precipitate the antibody-bound fraction. After aspiration of the unbound fraction, the antibody bound fraction of 125I-melatonin is counted. See instructions for use for references.
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