The Direct Saliva Melatonin RIA kit is intended for the direct quantitative determination of melatonin in human saliva samples. Research Use Only. Not for Use in Diagnostic Procedures.
The Direct Saliva Melatonin RIA Kit measures melatonin by a double-antibody radio immunoassay based on the Kennaway G280 anti-melatonin antibody (5,6). Human saliva samples and reconstituted standards and controls are incubated with the anti-melatonin antibody and 125I-melatonin. 125I-melatonin competes with melatonin present in samples, standards and controls. After 20 hours of incubation, solid-phase second antibody is added to the mixture in order to precipitate the antibody-bound fraction. After aspiration of the unbound fraction, the antibody bound fraction of 125I-melatonin is counted. See instructions for use for references.
Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
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