Anti-Phospholipid-8 IgG/IgM EIA is a solid phase enzyme immunoassay for the separate qualitative detection of IgG and/or IgM antibodies against phospholipids in human serum. The assay employs highly purified human ß2-Glycoprotein I, Cardiolipin + ß2-Glycoprotein I, Cardiolipin and Phosphatidylcholine, -ethanolamine, -inositol, -serine, and Sphingomyelin. Research Use Only. Not for Use in Diagnostic Procedures. procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
Cut-off
Sensitivity
n/a
Sizes
96 Wells
Sample Types
Serum
Inc Time Hour
1
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
Serum samples diluted 1:101 are incubated in microplates coated with the specific antigen.
Antibodies, if present in the sample, bind to the antigen. The unbound fraction is washed off in the
following step. Afterwards antihuman immunoglobulins conjugated to horseradish peroxidase
(conjugate) are incubated and react with the antigen-antibody complex of the samples in the
microplates. Unbound conjugate is washed off in the following step. Addition of TMB substrate
generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes
to yellow). The intensity of color formed from the reaction is a function of the amount of conjugate
bound to the antigen-antibody complex and is proportional to the initial concentration of the respective
antibodies in the sample.
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