Alpha 1 Antitrypsin ELISA (Clearance)
The Alpha-1 Antitrypsin ELISA is intended for the quantitative determination of alpha-1 antitrypsin (α1-antitrypsin) in human stool samples. The Alpha 1 Antitrypsin ELISA (Clearance) kit recognizes the hepatic and enteral a-1-antitrypsin forms. Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Available in North America Only
Plasma, Serum, Stool
The assay utilizes the “sandwich” technique with two selected antibodies that bind to human α1-
antitrypsin. Standards, controls, and prediluted samples, which are assayed for human α1-antitrypsin,
are added into the wells of a microplate coated with a high affinity anti-human α1-antitrypsin antibody.
During the first incubation step, α1-antitrypsin is bound by the immobilized antibody. Then a
peroxidase-conjugated polyclonal anti-human α1-antitrypsin antibody is added into each microtiter
well and a “sandwich” of capture antibody–human α1-antitrypsin–peroxidase-conjugate is formed.
Tetramethylbenzidine is used as peroxidase substrate. Finally, an acidic stop solution is added to
terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is
directly proportional to the concentration of α1-antitrypsin. A dose response curve of the absorbance
(optical density, OD at 450nm) versus concentration is generated, using the values obtained from
the standards. The α1-Antitrypsin present in the samples is determined directly from this curve.