The Ultrasensitive Human Growth Hormone ELISA is an enzyme immunoassay for the high-sensitive quantitative determination of growth hormone in human serum and plasma (heparin, EDTA) samples.
Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
50 - 1000 pg/mL
Sensitivity
1.6 pg/mL
Sizes
96 Wells
Sample Types
EDTA Plasma, Heparin Plasma, Plasma, Serum
Inc Time Hour
3
Inc Time Minute
15
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
The Ultrasensitive Human Growth Hormone ELISA is a sandwich assay. It utilizes a specific, high affinity polyclonal rabbit antiserum coated on the wells of a microtiter plate. The hGH in the samples binds quantitatively to the immobilized antiserum. In the following step, the biotinylated antibody in turn binds to hGH. After washing, a streptavidin-peroxidase-enzyme conjugate is added, which binds with high specificity to the biotin of the antibody and will catalyze the substrate to change color quantitatively depending on the hGH level of the sample.
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