Ultrasensitive Corticosterone ELISA is for the quantitative detection of Corticosterone in biological fluids. Research Use Only. Not for Use in Diagnostic Procedures.
The Corticosterone Enzyme-Linked Immunosorbent Assay (ELISA) kit is a competitive
immunoassay for the quantitative determination of Corticosterone in biological fluids. The kit uses a polyclonal antibody to
Corticosterone to bind, in a competitive manner, Corticosterone in the standard or sample or
an alkaline phosphatase molecule which has Corticosterone covalently attached to it. After a
simultaneous incubation at room temperature the excess reagents are washed away and
substrate is added. After a short incubation time the enzyme reaction is stopped and the
yellow color generated is read on a microplate reader at 405nm. The intensity of the bound
yellow color is inversely proportional to the concentration of Corticosterone in either standards
or samples. The measured optical density is used to calculate the concentration of
Corticosterone.
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