cGMP Complete ELISA 5 Plate

The cGMP complete Enzyme-linked Immunosorbent Assay (ELISA) kit is a competitive immunoassay for the quantitative determination of cyclic GMP in cells and tissue treated with 0.1M HCl, in addition to culture supernatants, urine, saliva, and serum. The optional acetylated assay format provides an approximate 10-fold increase in sensitivity and is ideal for samples with extremely low levels of cGMP. If expected levels of cGMP are unknown, the investigator may evaluate a few samples in the non-acetylated format to determine if higher sensitivity is required. In the cGMP Complete ELISA, standards and samples are added to wells coated with a GxR IgG antibody. A blue solution of cGMP conjugated to alkaline phosphatase is then added, followed by a yellow solution of rabbit polyclonal antibody to cGMP. During a simultaneous incubation at room temperature the antibody binds, in a competitive manner, the cGMP in the sample or conjugate. The plate is washed, leaving only bound cGMP. pNpp substrate solution is added. The substrate generates a yellow color when catalyzed by the alkaline phosphatase on the cGMP conjugate. Stop solution is added. Absorbance is read at 405nm. The amount of signal is indirectly proportional to the amount of cGMP in the sample.