The Biotin Microbiological Assay is a microtiter plate test kit based on a microbiological assay which measures the total biotin content in serum. The test kit contains all required reagents, e.g., standard, medium, and microtiter plate coated with a specific microorganism, sufficient for 96 determinations including standard curves. An ELISA reader is required for the evaluation of the biotin content. Research Use Only. Not for Use in Diagnostic Procedures.
Biotin (vitamin H) is widespread in bacteria, fungi, higher plants and animal tissues. The major part of biotin in food is covalently bound to protein with only a small part being freely available. During digestion, biocytin (biotinyl-lysin) is released from the proteins and can, similarly to biotin, easily be taken up from the intestine. Biotin is then released by the enzyme biocytinase from biocytin in plasma and erythrocytes and is available as prosthetic group for several biotin-dependent enzymes.
Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
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