Rat NT-proBNP ELISA
$731.00
Catalog
04-BI-1204R
The Rat NT-proBNP ELISA kit is for the quantitative determination of rat N-Terminal pro-Brain Natriuretic Peptide (NT-proBNP) in serum or plasma. For research use only. Not for use in diagnostic procedures.
In a first step, ASYBUF (Assay buffer), STD/CTRL/Sample (Standard, Control, Sample) and detection antibody (biotinylated polyclonal anti-rat NT-proBNP antibody, AB) are pipetted into
the respective wells of the microtiter strips, which are pre-coated with a polyclonal anti-rat
NT-proBNP antibody. Rat NT-proBNP present in the sample binds to the pre-coated antibody
in the well and forms a sandwich with the detection antibody.
After a first wash step, which removes non-specifically unbound material, the conjugate
(Streptavidin-HRPO) is added and reacts with the detection antibody. After another washing
step, the substrate (TMB, tetramethylbenzidine) is pipetted into the wells.
The enzyme catalysed color change of the substrate is directly proportional to the amount
of rat NT-proBNP present in the sample. This color change is detectable with a standard
microtiter plate ELISA reader. The concentration of rat NT-proBNP in the sample is determined directly from the dose response curve. The kit utilizes recombinant rat NT-proBNP as a
calibrator.
Species
Rat
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
50 - 3,200 pg/mL
Sensitivity
21 pg/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Hour
3
Inc Time Minute
30
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
