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Mouse KIM-1 ELISA

$759.00
Catalog
41-KIMMS-E01

The Mouse KIM-1 ELISA is a highly sensitive two-site enzyme linked immunoassay for measuring KIM-1 in biological samples of mice.

Research Use Only. Not for Use in Diagnostic Procedures.
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Mouse KIM-1 ELISA Instructions for Use
Mouse KIM-1 ELISA Instructions for Use
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Mouse KIM-1 ELISA Safety Data Sheet
Mouse KIM-1 ELISA Safety Data Sheet
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Species
Mouse
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
0.078 - 5 ng/mL
Sensitivity
0.078 ng/mL
Sizes
96 Wells
Sample Types
Serum, Urine
Inc Time Hour
2
Inc Time Minute
40
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
100
Detection
Colorimetric

KIM-1 is a trans-membrane structural glycoprotein in renal epithelial cells. These cells will shed KIM-1 antigen during regeneration into the urine which makes urinary KIM-1 a useful biomarker for kidney injury.

In this assay, the KIM-1 present in samples reacts with the anti-KIM-1 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-KIM-1 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound KIM-1. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of KIM-1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of KIM-1 in the test sample. The quantity of KIM-1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Mouse KIM-1 ELISA

41-KIMMS-E01

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