Metanephrine ELISA
$514.00
Catalog
17-MTPHU-E01
The Metanephrine ELISA kit is a quantitative immunoassay for the determination of metanephrine in human urine. For research use only. Not for use in diagnostic procedures.
Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
10.5 - 2,000 ng/ml
Sensitivity
10.5 ng/ml
Sizes
96 Wells
Sample Types
Urine
Inc Time Hour
1
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
25
Detection
Colorimetric
During the sample preparation metanephrine (metadrenaline) is quantitatively acylated. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations.