Intact Parathyroid Hormone (PTH) Immunoradiometric Assay (IRMA)
$611.00
Catalog
38-KIR1491
Immunoradiometric assay kit for the quantitative measurement of intact human Parathyroid Hormone (PTH) in serum and plasma. This two-step IRMA is highly specific for the intact h-PTH and does not cross react with active and inactive fragments even at high concentrations as suggested by HACKENG and al. Research Use Only. Not for Use in Diagnostic Procedures. Replacement for product 72-ELSA-PTH.
Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in USA Only
Range
0.7 - 1500 pg/ml
Sensitivity
0.7 pg/ml
Sizes
100 Tubes
Sample Types
EDTA Plasma, Serum
Inc Time Hour
18
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
200
Detection
I-125
Human parathyroid hormone (hPTH) is a major physiological regulator of phosphocalcic metabolism.
hPTH increases serum calcium concentrations by its actions on kidney (enhancing tubular Ca++ reabsorption
and phosphate excretion) and bone (stimulating osteoclastic activity and bone resorption). It indirectly
affects intestinal absorption of Ca++ by stimulating renal 1α-hydroxylation of 25 hydroxyvitamin D. The
release of PTH is controlled in a negative feedback loop by the serum concentration of Ca++.
PTH is synthesized in the chief cells of the parathyroid glands and secreted as an 84 amino acid molecule
called "intact PTH", which is the main bioactive product. This molecule is degraded by proteolytic cleavage
between amino acids 33-37 at peripheral sites to form biologically active amino-terminal fragments and
biologically inactive carboxyl-terminal fragments. The carboxyl-terminal fragments are cleared only by
glomerular filtration, while the bioactive intact PTH and amino-terminal fragments are also metabolically
degraded in the liver and other tissues. The half-life of the carboxyl-terminal fragments increases
dramatically in patients with renal failure. Thus, the measurement of intact PTH correlates best with the
hormone production and biological activity.