The Total Proinsulin ELISA is a quantitative immunoassay for the determination of total proinsulin (intact proinsulin, 32-33 split proinsulin, and des 31-32 split proinsulin) in human serum, EDTA plasma, and heparin plasma samples. For Research Use Only. Not for use in diagnostic procedures.
Proinsulin is a precursor molecule for insulin and is synthesised by the pancreatic β-cells. Under normal circumstances, virtually all proinsulin is cleaved at residues 32-33 and 65-66 to produce insulin during the formation of secretory granules. Some proinsulin is released into the circulation, mainly as des 31-32 split proinsulin together with lesser amounts of 32-33 split proinsulin and intact proinsulin. It is generally believed that 65-66 split proinsulin and des 64-65 split proinsulin do not appear in detectable amounts in the circulation. Increased concentrations of circulating proinsulin may occur in non-insulin dependent (type II) diabetes and in patients with insulinoma. When used in conjunction with a specific intact proinsulin assay, measurements of total proinsulin can provide useful information on changes in the processing of insulin in such situations.
1. What percentage of your IgE allergy testing panel consists of molecular allergens rather than allergen extracts? The ALEX2 IgE allergen panel consists of 117 extracts and 183 molecular allergens. This represents the largest panel of molecular allergens on the market, including 50 unique...
Cases of food and environmental allergies have been increasing over the last decade. While conservative estimates suggest IgE-mediated allergy affects 6-8% of children and 2-3% of adults, some estimates are as high as 30%.3, 2 Symptoms can range from mild to severe and include itching, hives,...
Food sensitivity or food intolerance involves a non-allergic response to a food or food additive. In a recent on-line survey conducted in the U.S., 24% of adults aged 18 to 79 reported a food intolerance. While a food allergy is typically mediated by IgE antibodies, adverse reactions to food...
In a head-to-head comparison of FDA-cleared assays and a novel ALPCO method, investigators observed wide variability in specificity and false-positive rates across platforms, raising important questions about standardization and clinical interpretation.
July 22, 2025 – Salem, NH – NuvinkaDx, a leading innovator in diagnostic solutions, today announced two significant product and regulatory milestones from its operating companies, GeneProof and ALPCO.
Energy homeostasis is a focus of research into diabetes and obesity. Read about how our assays in this area can assist both academic and industry researchers.
Complement System Solutions for Drug Development and Clinical Research Datasheet
The complement system is essential to innate immunity from a variety of diseases. ALPCO’s full range of complement system immunoassays can drive discovery.
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