Hepatitis A Virus Antigen ELISA is for the qualitative determination of Hepatitis A Virus (HAV) Antigen in human stool and cell culture supernatant. Research Use Only. Not for Use in Diagnostic Procedures.
Samples are pipetted into wells of a microplate coated with antibodies directed against Hepatitis A Virus (HAV). The HAV antigen binds to the fixed antibody and after the incubation period of two hours at 37°C the plate is washed thoroughly. Bound HAV antigen is identified with conjugate addition (monoclonal anti-HAV, peroxidase conjugated), incubated for another two hours at 37°C. Excess conjugate is removed by washing and the substrate is added. After 30 minutes incubation at room temperature the reaction is terminated by addition of stop solution. The blue color of a positive reaction turns to yellow and is measured in a microplate reader at 450 nm. The intensity of the color indicates the concentration of bound HAV antigen. A positive reaction must be confirmed by re-testing with anti-HAV neutralizing serum to discriminate false positive reactions which sometimes occur in stool.
1. What percentage of your IgE allergy testing panel consists of molecular allergens rather than allergen extracts? The ALEX2 IgE allergen panel consists of 117 extracts and 183 molecular allergens. This represents the largest panel of molecular allergens on the market, including 50 unique...
Cases of food and environmental allergies have been increasing over the last decade. While conservative estimates suggest IgE-mediated allergy affects 6-8% of children and 2-3% of adults, some estimates are as high as 30%.3, 2 Symptoms can range from mild to severe and include itching, hives,...
Food sensitivity or food intolerance involves a non-allergic response to a food or food additive. In a recent on-line survey conducted in the U.S., 24% of adults aged 18 to 79 reported a food intolerance. While a food allergy is typically mediated by IgE antibodies, adverse reactions to food...
In a head-to-head comparison of FDA-cleared assays and a novel ALPCO method, investigators observed wide variability in specificity and false-positive rates across platforms, raising important questions about standardization and clinical interpretation.
July 22, 2025 – Salem, NH – NuvinkaDx, a leading innovator in diagnostic solutions, today announced two significant product and regulatory milestones from its operating companies, GeneProof and ALPCO.
Energy homeostasis is a focus of research into diabetes and obesity. Read about how our assays in this area can assist both academic and industry researchers.
Complement System Solutions for Drug Development and Clinical Research Datasheet
The complement system is essential to innate immunity from a variety of diseases. ALPCO’s full range of complement system immunoassays can drive discovery.
Melatonin Products for Sleep Disorder-Related Research Datasheet
The melatonin hormone affects the circadian rhythm and sleep-wake cycle. Find out about our immunoassays and products that explore melatonin and sleep disorders.
