The Human Growth Hormone Binding Protein ELISA kit is for the quantitative measurement of human Growth Hormone Binding Protein levels in human serum or plasma.
Sandwich Assay Format
Colormetric Detection Controls and Standards
Small Sample Volume
Flexible Sample Type
Research Use Only. Not for Use in Diagnostic Procedures.
The assay is based on polyclonal antibodies and recombinant GHBP, expressed in eukaryotic cells.The ELISA for GHBP is a so-called Sandwich-Assay. It utilizes two specific antibodies of high affinity. First, the GHBP in the sample binds to the immobilized antibody on the microtiter plate. In a two-step sequence, the biotin-conjugated anti-GHBP-Antibody and the streptavidin-peroxidase are bound. Subsequently, the peroxidase catalyzes an enzymatic reaction resulting in a blue coloration. The intensity of the blue color depends on the GHBP content of the sample. The reaction is stopped by the addition of stop solution and color intensity is quantified by measuring the absorption. The GHBP ELISA allows secure and reproducible measurement of GHBP in human body fluids and is a suitable tool for the investigation of GHBP as biomarker in energy and fat metabolism.
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