The Glutamic Acid Decarboxylase ELISA (GAD ELISA) kit is an in vitro qualitative ELISA test for the detection of circulating autoantibodies to glutamic acid decarboxylase (GAD) antigen. Research Use Only. Not for Use in Diagnostic Procedures.
A purified GAD antigen is immobilized onto microwells. GAD specific IgG antibodies present in the serum sample are allowed to react with the antigen. The excess/unbound serum proteins are washed-off from the microwells. An enzyme (alkaline phosphatase) labeled goat antibody, specific to human IgG, is added to the GAD-antibody complex. After washing off excess unreacted enzyme conjugate from the microwells, a substrate (PNPP) is added and the color generated is measured spectrophotometrically. The intensity of the color developed directly gives the concentration of GAD autoantibodies in the test serum sample. GAD positive and negative controls serve as an internal quality control to ensure valid results.
1. What percentage of your IgE allergy testing panel consists of molecular allergens rather than allergen extracts? The ALEX2 IgE allergen panel consists of 117 extracts and 183 molecular allergens. This represents the largest panel of molecular allergens on the market, including 50 unique...
Cases of food and environmental allergies have been increasing over the last decade. While conservative estimates suggest IgE-mediated allergy affects 6-8% of children and 2-3% of adults, some estimates are as high as 30%.3, 2 Symptoms can range from mild to severe and include itching, hives,...
Food sensitivity or food intolerance involves a non-allergic response to a food or food additive. In a recent on-line survey conducted in the U.S., 24% of adults aged 18 to 79 reported a food intolerance. While a food allergy is typically mediated by IgE antibodies, adverse reactions to food...
In a head-to-head comparison of FDA-cleared assays and a novel ALPCO method, investigators observed wide variability in specificity and false-positive rates across platforms, raising important questions about standardization and clinical interpretation.
July 22, 2025 – Salem, NH – NuvinkaDx, a leading innovator in diagnostic solutions, today announced two significant product and regulatory milestones from its operating companies, GeneProof and ALPCO.
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