GLP-2 ELISA

The GLP-2 ELISA is based on a competitive enzyme immunoassay using a combination of highly specific antibody to human GLP-2 and the biotin-avidin affinity system. Standards, samples, biotinylated human GLP-2, and rabbit anti-GLP-2 antibody are added to the microplate wells coated in goat anti-rabbit IgG for the competitive immunoreaction. After incubation and plate washing, horse radish peroxidase (HRP)-labeled streptavidin (SA) is added to form an HRP-labeled SA - biotinylated GLP-2 - antibody complex on the surface of the wells. Finally, HRP enzyme activity is determined by o-phenylenediamine dihydrochloride (OPD) and the concentration of human GLP-2 is calculated. The proglucagon gene is expressed in both pancreatic A cells and intestinal L cells. Tissue-specific posttranslational processing of proglucagon by prohormone convertase produces different proglucagon derived peptides (PGDPs) in both the pancreas and intestine. The most notable pancreatic PGDP is glucagon, whereas intestinal L cells produce several structurally related peptides, including glucagon-like peptide 1 (GLP-1) and 2 (GLP-2), as well as glicentin and oxyntomodulin which contain the glucagon sequence in their molecules. Among PGDPs, GLP-2 has been found to result in intestinal epithelial proliferation.