Etanercept Drug Level ELISA
Catalog
30-ETLHU-E01
The Etanercept Drug Level ELISA is intended for the quantitative determination of free Enbrel® concentration in human serum and EDTA plasma. Research Use Only. Not for Use in Diagnostic Procedures.
Item 30-ETLHU-E01 is no longer available. An automated CLIA assay is coming soon. To receive updates on the replacement product, please submit this brief form.
This ELISA is designed to determine the quantity of free etanercept (therapeutic antibody against TNFα, ENBREL®) in EDTA plasma or serum samples. In a first incubation step, the free etanercept from the sample is bound to the specific monoclonal anti-etanercept antibody coated on the plate. To remove all unbound substances, a washing step is carried out. In a further incubation step, peroxidase-labeled antibody is added. Tetramethylbenzidine (TMB) is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of free etanercept in the sample. A dose response curve of the absorbance unit (optical density, OD) vs. concentration is generated, using the values obtained from the standards. The concentrations of free etanercept in the samples are determined directly from this curve.
Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
4.15-225 ng/mL
Sensitivity
2.88 ng/mL
Sizes
96 Wells
Sample Types
EDTA Plasma, Serum
Inc Time Hour
2
Inc Time Minute
20
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
