Ceruloplasmin ELISA
$590.00
Catalog
41-CERHU-E01
Ceruloplasmin ELISA is for the quantitative determination of Ceruloplasmin in human plasma and serum samples. Research Use Only. Not for Use in Diagnostic Procedures.
In this assay the
ceruloplasmin (Cp) present in samples reacts with the anti-Cp antibodies which have been adsorbed
to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, the
Detection Antibody, biotin conjugated anti-Cp, is added and complexes are formed. Following a
wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added, and complexes are
formed. After another washing step, the complexes are assayed by the addition of a chromogenic
substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with
the concentration of Cp in the sample tested; thus, the absorbance, at 450 nm, is a measure of the
concentration of Cp in the test sample. The quantity of Cp in the test sample can be interpolated
from the standard curve constructed from the standards and corrected for sample dilution.
Species
Human
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
3.1 ng/ml - 200 ng/ml
Sensitivity
3.1 ng/ml
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Hour
1
Inc Time Minute
40
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
