Anti-Pseudomonas aeruginosa IgG ELISA is for the qualitative or semi-quantitative detection of anti-pseudomonas aeruginosa immunoglobulin G (IgG) in human serum. Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Isotype
IgG
Range
Cut off Assay
Sensitivity
Cut off Assay
Sizes
Other
Size Other
3 x 96 Wells
Sample Types
EDTA Plasma, Serum
Inc Time Hour
4
Inc Time Minute
30
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
The assay is a sandwich enzyme immunoassay. Serum or plasma samples are diluted and added to the wells of a microtiter plate, which have been previously coated with the Pseudomonas aeruginosa antigens alkaline protease, elastase, or exotoxin A. Specific antibodies in the sample bind to the respective antigens during an incubation of 2 h at 37°C. After washing, the conjugate (peroxidase-labelled anti-human IgG immunoglobulin) is added and incubated again for 2 h at 37°C. After a final washing step, substrate is added and incubated for 30 min at room temperature. The color forming reaction is terminated on addition of stop solution accompanied by a color change from blue to yellow. The absorbance of the colored reaction product is measured on a microtiter plate reader. The color intensity of the reaction corresponds to the concentration of antibodies in the sample.
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