Rat C-Peptide ELISA

$630.00
Catalog
80-CPTRT-E01

The Rat C-peptide ELISA quantifies the C-peptide protein products of rat I and rat II proinsulin genes.

  • Only 10 µL of sample is sufficient for the assay
  • Superior sensitivity of 10.8 pM
  • Dynamic range of 50-4,500 pM
Research Use Only. Not for Use in Diagnostic Procedures.
Species
Rat
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
50 - 4500 pM
Sensitivity
10.8 pM
Sizes
96 Wells
Sample Types
Serum
Inc Time Hour
2
Inc Time Minute
15
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
The ALPCO Rat C-peptide ELISA is a sandwich type immunoassay. The 96-well microplate is coated with a monoclonal antibody specific for C-peptide. The standards, controls, and samples are added to the microplate wells with the conjugate. The microplate is then incubated at room temperature on a microplate shaker at 700-900 rpm. After the first incubation is complete, the wells are washed with Wash Buffer and blotted dry. TMB Substrate is added, and the microplate is incubated a second time at room temperature on a microplate shaker at 700-900 rpm. Once the second incubation is complete, Stop Solution is added, and the optical density (OD) is measured by a spectrophotometer at 450 nm. The intensity of the color generated is directly proportional to the amount of C-peptide in the sample.

Rat C-Peptide ELISA

80-CPTRT-E01

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