Mouse IgG2B ELISA
$548.00
Catalog
41-I2BMS-E01
The Mouse IgG2B ELISA is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgG2B in biological samples of mice.
Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Species
Mouse
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
6.25-200 ng/mL
Sensitivity
6.25 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Hour
1
Inc Time Minute
15
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
In this assay the IgG2B
present in samples reacts with the anti-IgG2B
antibodies which have been adsorbed to the surface
of polystyrene microtitre wells. After the removal of
unbound proteins by washing, anti-IgG2B antibodies
conjugated with horseradish peroxidase (HRP), are
added. These enzyme-labeled antibodies form
complexes with the previously bound IgG2B.
Following another washing step, the enzyme bound
to the immunosorbent is assayed by the addition of a
chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine
(TMB). The quantity of bound enzyme varies directly
with the concentration of IgG2B in the sample tested;
thus, the absorbance, at 450 nm, is a measure of the
concentration of IgG2B in the test sample. The
quantity of IgG2B in the test sample can be
interpolated from the standard curve constructed
from the standards, and corrected for sample dilution.