Mouse IgG1 ELISA
$429.00
Catalog
41-IG1MS-E01
The Mouse IgG1 ELISA is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgG1 in biological samples of mice. Research Use Only. Not for Use in Diagnostic Procedures.
Species
Mouse
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
6.25-200 ng/mL
Sensitivity
6.25 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum
Inc Time Hour
1
Inc Time Minute
30
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
5
Detection
Colorimetric
In this assay the IgG1
present in samples reacts with the anti-IgG1 antibodies which have been adsorbed to the surface of
polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG1 antibodies
conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form
complexes with the previously bound IgG1. Following another washing step, the enzyme bound to the
immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine
(TMB). The quantity of bound enzyme varies directly with the concentration of IgG1 in the sample
tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG1 in the test sample.
The quantity of IgG1 in the test sample can be interpolated from the standard curve constructed from the
standards, and corrected for sample dilution.