Granzyme B ELISA

Granzyme B ELISA
Granzymes are exogenous serine proteinases (enzymes) that are released from cytoplasmic granules of cytotoxic lymphocytes (CTLs) and NK cells.

The name "granzymes" is derived from: granules + enzymes. These granules contain next to granzymes other proteins including a poreforming protein (Perforin). Upon binding of the CTL to a target cell (by CTL-receptor and antigen-presenting MHC molecules on the target cell) the contents of the granules are released in the intercellular space where after perforine will "perforate" the target cell membrane by forming transmembrane pores. Through these pores the granzymes can now enter the cytosol of the target cell. Granzyme B activates the intracellular cascade of caspases finally resulting in the killing of the target cells.

Percentages of the Granzyme A and B positive CTLs can be determined by flow cytometry and immunocytochemical methods for many disorders. Not all granzymes enter the target cell, part of them also "leak" in to the peripheral blood and other biological fluids. Detectable amounts of granzymes have been found to circulate in healthy volunteers. These soluble granzymes can be measured by ELISAs.

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Product: Granzyme B ELISA

Catalog#: 61-GRBHU-E01

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Additional Information

Catalog #
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
0.66 - 480 pg/mL
0.2 pg/mL
96 Wells
Sample Types
Cell Culture, Serum
Incubation Time
2 hr
Sample Size
50 µL
Instructions for Use
Safety Data Sheet
Additional Information
Additional Information
The human Granzyme B ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Granzyme B. The human Granzyme B ELISA is for research use only. Not for diagnostic or therapeutic procedures.

For more information on the Granzyme B ELISA, call 800-592-5726.
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