Free Estriol ELISA
$392.00
Catalog
20-FE3HU-E01
The Free Estriol ELISA is an enzyme immunoassay for the quantitative in vitro diagnostic measurement of free estriol (unconjugated estriol) in serum during the second half of pregnancy. FDA Registered. For In Vitro Diagnostic Use.
Species
Human
Regulatory Status
FDA Registered. For In Vitro Diagnostic Use.
Product Distribution
Available Worldwide
Range
0.3 - 40 ng/mL
Sensitivity
0.075 ng/mL
Sizes
96 Wells
Sample Types
Serum
Inc Time Hour
1
Inc Time Minute
30
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
The Free Estriol ELISA kit is a solid-phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with a polyclonal [rabbit] antibody directed towards an antigenic site on the Estriol molecule. Endogenous Estriol of a sample competes with an Estriol-horseradish peroxidase conjugate for binding to the coated antibody. After incubation, the unbound conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of Estriol in the sample. After the addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of Estriol in the sample.