The DHEA ELISA kit is a solid-phase enzyme-linked immunosorbent assay (ELISA), based onthe principle of competitive binding. The microtiter wells are coated with a polyclonal (rabbit)antibody directed toward an antigenic site on the DHEA molecule. During the first incubation,endogenous DHEA of a sample competes with a DHEA-horseradish peroxidase conjugate forbinding to the coated antibody. After incubation, the unbound conjugate is washed off, and thesolid phase is incubated with substrate solution. The colorimetric reaction is stopped by additionof stop solution, and the optical density (OD) of the resulting yellow product is measured. Theintensity of color is inversely proportional to the concentration of the analyte in the sample. Astandard curve is constructed by plotting OD values against concentrations of standards, andconcentrations of unknown samples are determined using this standard curve.
1. What percentage of your IgE allergy testing panel consists of molecular allergens rather than allergen extracts? The ALEX2 IgE allergen panel consists of 117 extracts and 183 molecular allergens. This represents the largest panel of molecular allergens on the market, including 50 unique...
Cases of food and environmental allergies have been increasing over the last decade. While conservative estimates suggest IgE-mediated allergy affects 6-8% of children and 2-3% of adults, some estimates are as high as 30%.3, 2 Symptoms can range from mild to severe and include itching, hives,...
Food sensitivity or food intolerance involves a non-allergic response to a food or food additive. In a recent on-line survey conducted in the U.S., 24% of adults aged 18 to 79 reported a food intolerance. While a food allergy is typically mediated by IgE antibodies, adverse reactions to food...
ALPCO offers targeted GI solutions suitable for all types of labs. Our offerings range from plate-based to clinical chemistry analyzer-based and automated flash chemiluminescence solutions, all designed to accommodate your lab’s expanding testing needs and capabilities.
In the webinar, Dr. Benjamin Click from the University of Colorado, Department of Gastroenterology, underscores the critical role of non-invasive monitoring methods, particularly fecal calprotectin monitoring, in guiding treatment adjustments and achieving therapeutic goals..
ALPCO’s Active GLP-1 (7-36) amide Chemiluminescence ELISA accurately measures the major biologically active circulating form of GLP-1.1,2 This well-characterized assay provides high analytical sensitivity, a short incubation time, and measures active GLP-1 (7-36) amide in fasted and fed samples.
Energy homeostasis is a focus of research into diabetes and obesity. Read about how our assays in this area can assist both academic and industry researchers.
Complement System Solutions for Drug Development and Clinical Research Datasheet
The complement system is essential to innate immunity from a variety of diseases. ALPCO’s full range of complement system immunoassays can drive discovery.
Melatonin Products for Sleep Disorder-Related Research Datasheet
The melatonin hormone affects the circadian rhythm and sleep-wake cycle. Find out about our immunoassays and products that explore melatonin and sleep disorders.
