KIM-1 is a trans-membrane structural glycoprotein in renal epithelial cells. These cells will shed KIM-1 antigen during regeneration into the urine which makes urinary KIM-1 a useful biomarker for kidney injury.
Research Use Only. Not for Use in Diagnostic Procedures.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
0 - 4 ng/mL
Sensitivity
0.125 ng/mL
Sizes
96 Wells
Sample Types
Plasma, Serum, Urine
Inc Time Hour
1
Inc Time Minute
50
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
25
Sample Size 2
10
Detection
Colorimetric
In this assay, the KIM-1 present in samples reacts with the anti-KIM-1 antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, the detection antibody, biotin conjugated anti-KIM-1, is added and complexes are formed. Following washing step, the horseradish peroxidase (HRP) conjugated streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’- tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of KIM-1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of KIM-1 in the test sample. The quantity of KIM-1 in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
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