Calprotectin ELISA is for the quantitative determination of calprotectin in human stool. Health Canada Licensed , Research Use Only. Not for Use in Diagnostic Procedures.
Health Canada Licensed, Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Range
32.5 - 2100 μg/g
Sensitivity
32.5 μg/g
Sizes
96 Wells
Sample Types
Stool
Inc Time Hour
1
Inc Time Minute
20
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
15
Detection
Colorimetric
The assay utilizes the two-site “sandwich” technique with two selected monoclonal antibodies that bind to human calprotectin. Standards, controls, and diluted samples, which are assayed for human calprotectin, are added to wells of a microplate coated with a high affinity monoclonal antihuman calprotectin antibody. During the first incubation step, calprotectin in the samples is bound by the immobilized antibody. Then, a peroxidase labeled conjugate is added to each microtiter well and a “sandwich” complex of capture antibody-human calprotectin-peroxidase conjugate is formed. Tetramethylbenzidine (TMB) is used as peroxidase substrate. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the calprotectin concentration in the sample. A standard curve is generated using the values obtained from the standards. The calprotectin present in the samples is determined directly from this curve.
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