Bradykinin ELISA
$792.00
Catalog
74-BRAHU-E01
The Bradykinin ELISA kit is a colorimetric, competitive immunoassay kit with results in 3 hoursf for the quantitative determination of species independent Bradykinin in plasma, serum and urine samples. Research Use Only. Not for Use in Diagnostic Procedures.
Regulatory Status
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available Worldwide
Range
11.7-30,000 pg/mL
Sensitivity
24.8 pg/mL
Sizes
96 Wells
Sample Types
Plasma, Serum, Urine
Inc Time Hour
3
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
10
Detection
Colorimetric
Bradykinin was discovered in 1949 as a substance generated from a globulin precursor in plasma by the action of proteases. Its name indicates that it causes a slow movement of the gut1. As early as 1909 it was noted that substances found in urine, which were later identified as kinins, have hypotensive actions2. Kinins are effectors of vasodilation, vascular permeability, NO release and arachidonicacid mobilization. They are important regulators of blood pressure, kidney function and heart function, and they are also involved in inflammation2,3,5. Bradykinin is generated from the blood globulin kininogen HK, by the action of the kallikrein system in blood (related to the blood clotting cascade) but can also be generated in other tissues and organs3. Besides kallikrein, other proteases such as plasmin may also release bradykinin. Several peptidases can degrade kinins, including Angiotensin. See instructions for use for references.
Converting Enzyme (ACE), a metalloproteinase which converts Angiotensin I to Angio‐
tensin II and destroys bradykinin3,5. Plasma Bradykinin is rapidly degraded to a smaller
stable peptide (BK1‐5) form4
