The Anti-Transglutaminase Antibodies ELISA is intended for the quantitative measurement of anti-transglutaminase antibodies in stool samples. This ELISA is one of twelve that utilizes a single stool extraction procedure for a multi-analyte gastroenterology panel.
Research Use Only. Not for Use in Diagnostic Procedures.
Product Distribution
Available in North America Only
Sizes
96 Wells
Sample Types
Stool
Inc Time Hour
2
Inc Time Minute
20
Inc Time Overnight
No
Inc Time See Protocol
No
Sample Size
15
Sample Size 2
100
Detection
Colorimetric
This ELISA is used for the quantitative determination of anti-human tissue transglutaminase (antihtTG) sIgA antibodies in stool. In a first incubation step, the anti-htTG sIgA antibodies in the sample are bound to their antigen (human recombinant transglutaminase), which is immobilized to the surface of the microtiter plates. To remove all unbound foreign substances, a washing step is carried out. In a second incubation step, a peroxidase-labeled anti-sIgA antibody is added. After another washing step, to remove all unbound antibodies, the solid phase is incubated with the substrate, tetramethylbenzidine. An acidic stop solution is then added to stop the reaction. The color converts from blue to yellow. The intensity of the yellow color is directly proportional to the amount of bound anti-tTG antibodies and can be determined photometrically at 450 nm. The sample results are evaluated by comparison with a cut-off value.
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