1,25 (OH)2 Vitamin D Single-step Immunoextraction Method

A thumbnail preview of the 1,25(OH)2 Vitamin D Single-step Immunoextraction Method handout.

Vitamin D deficiency is estimated to affect 42% of American adults1 and is considered a major worldwide health issue2. The bioactive form of vitamin D known as 1,25-dihydroxyvitamin D [1,25(OH)2D] can be challenging to quantify accurately due to its picomolar concentrations in circulation, short half-life, and lipophilic nature2. Additionally, the extraction of 1,25(OH)2D is inhibited by binding proteins and vitamin D metabolites2,3. Therefore, sensitive and specific LC-MS/MS methodology is considered the gold standard for measuring 1,25(OH)2D.

The 1,25(OH)2D ImmunoTubes® offer a simple one-step extraction method, making in-house testing a possibility.

  • No derivatization, precipitation or SPE-extraction
  • No ion suppression
  • No interfering matrix components
  • No requirement for high-end instruments
  • No radioactive waste
  • No organic solutions

1. Forrest & Stuhldreher. (2011). Prevalence and correlates of vitamin D deficiency in US adults. Nutr Res., 31(1):48-54. doi: 10.1016/j.nutres.2010.12.001. PMID: 21310306.
2. Zitterman et al. (2016). Measurement of circulating 1,25-dihydroxyvitamin D: Comparison of an automated method with a liquid chromatography tandem mass spectrometry method. Int. J. Anal. Chem., 2016. 8501435. doi: 10.1155/2016/8501435. PMCID: PMC4835639.
3. Hollis & Horsta. (2007). The assessment of circulating 25(OH)D and 1,25(OH)2D: Where we are and where we are going. J. Steroid Biochem. Mol. Biol., 103(3-5). 473–476.