For Research Use Only

Literature References:

Product Specifications
sa
9/4/2009
12:10:53 PM

 

Summary:

* = The recommended starting sample volume in this kit is between 5 and 10 µl. See package insert for details (Section 7: Sample Volume Adjustment).

Adiponectin circulates as multiple isoforms¹ and is widely recognized as a key protein involved in insulin regulation and metabolic syndrome. Of the different multimers, the high-molecular-weight (HMW) form of adiponectin activates adenosine monophosphate activated protein kinase phosphorylation most effectively¹, and HMW levels, or the ratio of HMW to total adiponectin, are more meaningful than the total adiponectin level for predicting insulin resistance and the development of metabolic syndrome².

Recently, an ELISA system was developed for the selective quantification of human adiponectin oligomers using protease pretreatment³, and this kit reflects a similar system for simultaneous detection of mouse HMW and total adiponectin. ⁴

1) Hada Y, et al. Biochem Biophys Res Commun 2007; 356: 487-93.
2) Hara K, et al. Diabetes Care 2006; 29: 1357-62
3) Ebinuma H, et al. Clin Chim Acta 2006; 372: 47-53
4) Ebinuma H, et al. Clin Chim Acta 2009; 401: 181-3