For Research Use Only

 

Summary:

This highly sensitive GLP-1 ELISA (enzyme-linked immunosorbent assay) kit is produced exclusively for the quantitative determination of bioactive glucagon like peptide-1 (7-36) [GLP-1 (7-36)] levels in human EDTA-plasma and serum.

Assay standards, controls, and test samples are added directly to the wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 (7-36) specific antibody and a horseradish peroxidate (HRP) conjugated GLP-1 (7-36) specific antibody is added to each well. After the first incubation period, a sandwich immunocomplex of Streptavidin - Biotin-Antibody - GLP-1 (7-36) - HRP conjugated antibody forms attached to the walls of the plate. The unbound HRP conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP-1 (7-36) on the walls of the microplate wells is directly proportional to the amount of GLP-1 (7-36) in the sample.