For Research Use Only

 

Summary:

This sandwich ELISA is designed for the quantitative determination of underglycosylated Bone Sialoprotein in Serum.

The test principle is based on interaction of the antigen in the samples or standards with the antibody coated on the Wells of the microplate. A peroxidase-conjugated secondary antibody is used for detection and quantification, and tetramethylbenzidine (TMB) as a peroxidase substrate. The enzymatic reaction is terminated by an acidic stop solution. A dose response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standards. The quantity of Bone Sialoprotein present in the samples is determined directly from this curve.