Alpco assay

Ghrelin, Non-Acylated (Mouse/Rat) ELISA

ALPCO Catalog Number: 32-5118

Ghrelin, Non-Acylated (Mouse/Rat) ELISA

For Research Use Only This item appears in: Diabetes & Obesity Animal Assays  Format:  ELISA / EIA  Sample Type: EDTA Plasma  Sample Size: 10 µl  Size:  96 Wells  Range:  1.95 - 250 pg/ml  Sensitivity:  0.8 pg/ml  Incubation Time:  3 Hour  Protocol:    Download Protocol   (123 KB) International Distribution:  Only available in North America  Price:   Related Products:

Ghrelin is present in the peripheral circulation in two forms: acylated and non-acylated. This ELISA specifically measures the acylated form of ghrelin. This ELISA is based on a double-antibody sandwich technique; the Wells of the plate supplied with the kit are coated with a monoclonal antibody specific to the C-terminal part of ghrelin. This antibody will bind to any ghrelin introduced into the wells (standard or sample). The AChE - Fab` conjugate which recognizes the N-terminal part of acylated ghrelin is also added to the wells. This allows the two antibodies to form a sandwich by binding on different parts of the human acylated ghrelin. Each kit contains ghrelin (acylated) AChE-Fab` conjugate, human acylated ghrelin standard, human acylated ghrelin quality control, Ellman’s reagent, EIA buffer, wash buffer, Tween 20, plates pre-coated with anti-ghrelin mouse monoclonal antibody, and complete instructions.

Tags

adipokines octinylated Ghrelin, Ghrelin, Non-Acylated