Alpco ELISA

Bone Sialoprotein (BSP) ELISA

ALPCO Catalog Number: 30-4222
Bone Sialoprotein (BSP) ELISA
  For Research Use Only For Research Use Only
This item appears in:
Bone Metabolism
 Format:  ELISA / EIA
 Sample Type: serum
 Sample Size: 100 µl
 Size:  96 wells
 Range:  3.1 - 50 ng/ml
 Sensitivity:  1 ng/ml
 Incubation Time:  3 Hour
 Host:   0  
 Form:   0  
 Clone Isotype:   0  
 Known Applications:  0
 Protocol:   PDF Format Download Protocol   (569 KB)
International Distribution:  Only available in North America
 Price:  Buy Now
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This sandwich ELISA is designed for the quantitative determination of underglycosylated Bone Sialoprotein in Serum.

The test principle is based on interaction of the antigen in the samples or standards with the antibody coated on the Wells of the microplate. A peroxidase-conjugated secondary antibody is used for detection and quantification, and tetramethylbenzidine (TMB) as a peroxidase substrate. The enzymatic reaction is terminated by an acidic stop solution. A dose response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standards. The quantity of Bone Sialoprotein present in the samples is determined directly from this curve.

Tags

BSP, Bone Sialoprotein