The ALPCO Mouse Ultrasensitive Insulin ELISA kit quantifies the concentration of the insulin protein products of the mouse I and mouse II proinsulin genes from as little as 5µl of serum or plasma sample. Utilizing a dual-monoclonal antibody sandwich format, bi-level control set, and 96-well microplate comprising removable strips, a single Mouse Ultrasensitive Insulin ELISA kit has the performance characteristics and flexibility necessary to confidently measure up to 40 samples in duplicate. The shelf-life of the components and the resealable microplate pouch allow for convenient storage for future use if the entire kit is not needed at one time. Additional information regarding the Mouse Ultrasensitive Insulin ELISA kit can be accessed by clicking on the “Protocol” link or “Support” tab above.
The ALPCO Mouse Ultrasensitive Insulin ELISA is provided with seven standards designed to meet the needs of either very small sample size, or very low insulin concentrations. For small samples, such as tail snip samples, use a sample size of 5µl and the top five standards, 0.18-6.9 ng/ml. For hypoinsulinemic samples from a type 1 diabetes model or streptozotocin treatment, use a 25µl sample size and the bottom five standards, 0.025-1.25 ng/ml.
When more than 280 samples need to be assayed in duplicate, the ALPCO Mouse Ultrasensitive Insulin ELISA Jumbo/10-plate format is the economical option. Click on “Mouse Ultrasensitive Insulin ELISA Jumbo 80-INSMSU-E10” above and learn more about reducing the cost associated with quantifying insulin in a large number of samples.
If additional Zero Standard is required, click on the “Zero Standard” link in the “Related Product” section to order this product.